Journal: PLoS ONE

Article Title: Glucose-coated superparamagnetic iron oxide nanoparticles prepared by metal vapor synthesis can target GLUT1 overexpressing tumors: In vitro tests and in vivo preliminary assessment

doi: 10.1371/journal.pone.0269603

Figure Lengend Snippet: GLUT1 levels estimated by ELISA in PSN-1, BCPAP and HEK293 cells (at the top). Uptake studies: PSN-1 and BCPAP cells were incubated with 0.1 mg/mL of Glc-SPIONs for 30 min, 1, 3, 6, and 12 h. The Fe cellular content was estimated by GF-AAS analysis. Uptake studies after treating the cells with GLUT1 inhibitors: PSN1 and BCPAP cells were pre-incubated for 1 h with anti-GLUT1 polyclonal antibody, WZB117, Fasentin, BAY-876, and STF-31. Subsequently, cells were treated for 3 or 6 h with 0.1 mg/mL of Glc-SPIONs.

Article Snippet: Expression levels in cancer cells were evaluated by means of the GLUT1 Colorimetric Cell-Based ELISA kit (Boster Biological Technology, Pleasanton CA, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Incubation

Journal: iScience

Article Title: Lactate facilitates classical swine fever virus replication by enhancing cholesterol biosynthesis

doi: 10.1016/j.isci.2022.105353

Figure Lengend Snippet:

Article Snippet: Anti-SLC2A1 Antibody , BOSTER , RRID: AB_PB9435.

Techniques: Virus, Recombinant, Glucose Oxidase Assay, Lactate Assay, Cholesterol Assay, CCK-8 Assay, Bicinchoninic Acid Protein Assay, ATP Assay, Software

Journal: Journal of Neurochemistry

Article Title: Ethanol Alters DNMT 1/3a/3b Expression Profile, Promotes Persistent DNA Hypomethylation in Human Brain Endothelial Cells and Impairs Late Cortical Angiogenesis

doi: 10.1111/jnc.70375

Figure Lengend Snippet: Ethanol alters methylation levels of endothelial genes' promoter essential to BBB‐related functions. HBMEC cultures were treated with ethanol (50 or 100 mM) for 24 h, after which genomic DNA was isolated and methylated regions were immunoprecipitated by antibody binding (MeDIP). The immunoprecipitated DNA was subsequently analyzed by qPCR to assess methylation levels at specific gene promoters (a). Exposure to 50 mM ethanol did not significantly alter methylation levels at the Claudin‐5 promoter but significantly reduced methylation at the Glut1 promoter. Treatment with 100 mM ethanol significantly increased methylation at the Claudin‐5 promoter while reducing methylation at the Glut1 promoter (b). * p < 0.05, ** p < 0.01, *** p < 0.001 Data are presented as mean ± SEM. n = 3–4 independent cell culture preparations. HBMEC, human brain microcapillary endothelial cells; Glut1, glucose transporter 1.

Article Snippet: Among these, glucose transporter type 1 (GLUT1) is responsible for glucose delivery to the brain (Abbott ; Abbott et al. ).

Techniques: Methylation, Isolation, Immunoprecipitation, Binding Assay, Methylated DNA Immunoprecipitation, Cell Culture

Journal: Journal of Neurochemistry

Article Title: Ethanol Alters DNMT 1/3a/3b Expression Profile, Promotes Persistent DNA Hypomethylation in Human Brain Endothelial Cells and Impairs Late Cortical Angiogenesis

doi: 10.1111/jnc.70375

Figure Lengend Snippet: Impacts of ethanol exposure on brain endothelial cells and in a mouse model of PAE. (a) Schematic representation of blood–brain barrier (BBB) endothelial cells expressing basal levels of tight junction proteins, such as Claudin‐5, localized at the plasma membranes of neighboring cells. In the nuclei of human brain microcapillary endothelial cells (HBMECs) and mouse brain endothelial cells (MBECs) isolated from the prenatal alcohol exposure (PAE) mouse model, methylated DNA (5mC), DNA methyltransferases (DNMTs), methyl‐CpG binding protein 2 (MeCP2), and vascular endothelial zinc finger 1 (VEZF1) are depicted. (b) In the context of PAE, ethanol increases cortical vascular permeability, which is accompanied by reduced levels and altered spatial distribution of Claudin‐5 in newborn mice (P0). At later developmental stages (P22), vascular alterations persist, with reduced vascular arborization compared with control animals. In vitro, ethanol induces a global reduction in DNA methylation levels associated with decreased DNMT1 activity in HBMECs. In parallel, ethanol modulates the expression of DNMT3a/3b, MeCP2, and VEZF1, as well as the methylation status of promoter regions of glucose transporter 1 ( Glut‐1 ) and Claudin‐5 genes in a dose‐ and time‐dependent manner. Treatment with S‐adenosylmethionine (SAM) prevents ethanol‐induced DNA methylation changes in HBMECs. Created with BioRender .

Article Snippet: Among these, glucose transporter type 1 (GLUT1) is responsible for glucose delivery to the brain (Abbott ; Abbott et al. ).

Techniques: Expressing, Clinical Proteomics, Isolation, Methylation, Binding Assay, Permeability, Control, In Vitro, DNA Methylation Assay, Activity Assay